Composite

Part:BBa_K2121006:Design

Designed by: Leslie Wang   Group: iGEM16_Yale   (2016-10-21)


Arabinose Inducible Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4907
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal NotI site found at 2781
    Illegal NotI site found at 4314
    Illegal NotI site found at 5604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 682
    Illegal BamHI site found at 1489
    Illegal XhoI site found at 719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal NgoMIV site found at 251
    Illegal NgoMIV site found at 2226
    Illegal NgoMIV site found at 2400
    Illegal NgoMIV site found at 2450
    Illegal NgoMIV site found at 2454
    Illegal NgoMIV site found at 2481
    Illegal NgoMIV site found at 2980
    Illegal AgeI site found at 92
    Illegal AgeI site found at 5401
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5995
    Illegal SapI.rc site found at 5532


Design Notes

Utilize native suppression mechanism in S. meliloti.


Construct design Design_1.png

Design_2.png


Source

Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1.


References